Paul Diehl
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Articles by Paul Diehl

One Tube is all it Takes
Paul Diehl | | 3 min read
Novagen's STP3 performance with various templates Promega's TNT® system and Novagen's Single Tube Protein™ System 3 combine in vitro transcription and translation into a single-tube reaction. Traditionally, in vitro transcription and translation are carried out as two separate reactions where the RNA reactions is first synthesized using an in vitro transcription system and then added to a translation reaction, of which several are in use today. Carrying out two separate procedures c

Microscopy on the Fly
Paul Diehl | | 3 min read
CompuCyte's Laser Scanning Cytometer Brings Scanning Capabilities to Fluorescence Microscopy Anyone who has ever got a headache from staring into a microscope for an extended period to count fluorescently labeled cells will appreciate the utility of the Laser Scanning Cytometer LSC® rom CompuCyte. As a slide-based laser scanning instrument that offers the analytical and quantitative features of a flow cytometer, the LSC pioneers a new dimension in fluorescence microscopy. The LSC will automa

Biomolecular Interactions in Real Time with no Labeling
Paul Diehl | | 2 min read
Biacore offers instruments that identify biomolecular interactions and measure binding characteristics in real time. Schematic of biomolecular interaction analysis by Biacore's affinity based biosensors. Identifying and characterizing molecular interactions is becoming increasingly important in a number of areas of life science research. Membrane interactions between cells, ligand-receptor interactions involved with cell signaling, protein-protein interactions in signal transduction pathways,

More Than Just A Pretty Picture
Paul Diehl | | 9 min read
to keep up with ever expanding applications. Date: October 26, 1998Comparison of Products Ten years ago, a "gel documentation" system meant an instant camera on a pole over a transilluminator. As charged-couple device (CCD) cameras became less expensive, electronic hardware to create digital images became available, and sophisticated image enhancement and analysis software was developed, electronic gel documentation systems were introduced and have become an essential laboratory instrument for

Complete Absorption: No Problem With World Precision Instruments
Paul Diehl | | 3 min read
It's a very common problem: You need to determine the concentration of a molecule of interest spectrophotometrically but have only a few microliters of sample. Until now, there were not a lot of options. In the last year, World Precision Instruments has developed three instruments for just these sorts of microscale absorbence measurements. Dr. Su-Yi Liu, R&D scientist at World Precision Instruments, said that the company was originally formed "to develop instruments for electrophysiology bu

Biologically Active Peptides: Who Makes Them and Who Sells Them?
Paul Diehl | | 10+ min read
Date: September 14, 1998 Catalog Peptide Providers Table In the last 20 years or so, numerous peptides with a diverse array of biological activities have been identified. In the same time period, the advent of automated peptide synthesis has made these reagents cheap and easy to produce in large quantities. These developments have created a new class of research reagents, known as biologically active peptides. Many biologically active peptides occur naturally; however, many others have been de

Dendrimer probes from Genisphere: Probes with 1000x More Sensitivity Dendrimer 3DNA Probes from Genisphere
Paul Diehl | | 3 min read
Dendrimer probes from Genisphere Genisphere claims that use of its 3DNA Probe 100 or 3DNA Probe 1000 systems with a standard oligonucleotide probe amplifies the signal of any common detection method by 100- or 1,000-fold, respectively. These systems effect this amplification by employing a "dendrimer" to construct a labeled-oligonucleotide-DNA complex during the labeling reaction. Dendrimers are large DNA matrices that have multiple single-stranded DNA ends. A dendrimer monomer consists of tw

Get Your Sunburn on the Beach, Not at the Lab Bench
Paul Diehl | | 3 min read
Anyone who has ever gotten irritated eyes or a mild burn from leaning over a UV transilluminator to cut out DNA bands from a gel does not have to be told that UV irradiation can be dangerous. Viewing fluorescently labeled DNA using even long-range UV irradiation can cause damage to the face and eyes of the researcher as well as the DNA samples. Now, Clare Chemical Research, a Colorado company started just 12 months ago, offers an alternative to UV transilluminators: the Dark Reader product line

96-Well Chromatography With The Multiscreen(R) Assay System
Paul Diehl | | 3 min read
Sample isolation and preparation often creates a bottleneck in the execution and automation of high throughput processes. To address this issue, Millipore has recently adapted its MultiScreen Assay System to perform chromatographic separations. This adaptation enables researchers to purify many samples simultaneously and provides a platform that facilitates automation. The MultiScreen approach has been used successfully to perform several types of chromatographic separation (for example, size
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