Do-It-Yourself Protein Arrays

Protein arrays are a relatively new technique for studying protein-protein interactions and profiling antibody binding and specificity, and in the past year a number of companies have released premade arrays. Now, Rockford, Ill.-based Pierce Biotechnology offers a new line of kits designed for researchers looking for a simple, convenient way to array their own proteins or antibodies. Pierce's DiscoverLight™ kits use a gridded nitrocellulose membrane that includes preprinted biotin and p

Written byAileen Constans
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Protein arrays are a relatively new technique for studying protein-protein interactions and profiling antibody binding and specificity, and in the past year a number of companies have released premade arrays. Now, Rockford, Ill.-based Pierce Biotechnology offers a new line of kits designed for researchers looking for a simple, convenient way to array their own proteins or antibodies.

Pierce's DiscoverLight™ kits use a gridded nitrocellulose membrane that includes preprinted biotin and peroxidase-based controls to facilitate assay validation and array orientation. Marketing manager Mary Beth Anderson explains that the membrane is designed to allow users to segment the array into smaller sections if desired. "Customers can use either a full membrane or they can section out smaller ones depending upon how many samples they want to test at the same time. There's quite a bit of flexibility," she says, adding that the system is designed for low-density applications.

Pierce sells four versions ...

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