Cells transfected using FuGENE 6 reagent and protocol supplied by Roche Molecular Biochemicals. MCF-10A cells transfected with a GFP construct (pGreenLanternn-1; Gibco/BRL) and fixed with formaldehyde and counter-stained with DAPI to reveal nuclei. GFP expression (green) was detected by fluorescence microscopy using a standard FITC filter set. DAPI staining (various shades of blue) was detected by fluorescence microscopy using a DAPI filter set. Transfected cells have a light blue (almost white) nucleus surrounded by green; the lighter blue (white) is due to an overlap in the two colors. Image submitted by Randall Marsh, University of Cincinnati College of Medicine. Your transfections aren't working. Maybe your current method kills most of your cells. Or maybe you're working with a reluctant cell line and want to boost your transfection efficiencies. Not only have you attempted the various methods available, but you've tried every trick in the book. Maybe it's time to give ...
Hey, Easy On Those Cells!
Cells transfected using FuGENE 6 reagent and protocol supplied by Roche Molecular Biochemicals. MCF-10A cells transfected with a GFP construct (pGreenLanternn-1; Gibco/BRL) and fixed with formaldehyde and counter-stained with DAPI to reveal nuclei. GFP expression (green) was detected by fluorescence microscopy using a standard FITC filter set. DAPI staining (various shades of blue) was detected by fluorescence microscopy using a DAPI filter set. Transfected cells have a light blue (almost whit
Written byShane Beck
| 3 min read
Register for free to listen to this article
Listen with Speechify
0:00
3:00
Interested in reading more?
Become a Member of
Receive full access to digital editions of The Scientist, as well as TS Digest, feature stories, more than 35 years of archives, and much more!
Already a member? Login Here
Add The Scientist as a preferred Google source to see more of our trusted coverage.
Meet the Author
- This person does not yet have a bio.View Full Profile
Published In
