During T-lymphocyte activation, signals from a number of cell-surface receptors must be integrated to ensure the appropriate genetic response. Non-dividing, primary T lymphocytes are notoriously difficult to transfect, presenting an experimental limitation to dissecting signaling mechanisms. In the October issue of
They optimized the conditions for transiently transfecting mouse thymocytes and human T cells. Using electroporation they achieved over 50% transfection rates while maintaining high cell viability; they then demonstrated that this transfection technology could be used to examine the effects of MAP kinase signaling.
Using multiparameter flow cytometry, Bell