Measuring Membrane Proteins with Mass-Sensitive Particle Tracking

Researchers will discuss a new mass photometry method that characterizes molecules in their native state on supported lipid bilayers.

Event Details:Measuring Membrane Proteins with Mass-Sensitive Particle TrackingDate(s):

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Wednesday, June 30, 2021
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Mass photometry is a revolutionary way to measure the mass of molecules in their native states. Early mass photometry experiments, developed and pioneered by Philipp Kukura from the University of Oxford, imaged molecules on solid glass surfaces. However, many macromolecules, such as membrane-associated proteins and integral membrane proteins, perform their most important functions and interact with reaction partners in lipid membranes. In this webinar brought to you by Refeyn, Nikolas Hundt, a former postdoctoral researcher in the Kukura laboratory, now at Ludwigs-Maximilian-University Munich, will describe a new mass photometry strategy for unlabelled molecules diffusing on supported lipid bilayers. With this approach, called mass-sensitive particle tracking (MSPT), researchers can determine the mass distributions and diffusion characteristics of membrane-associated protein complexes and observe protein assembly dynamics on a lipid interface in real time.

Nikolas Hundt will be ...

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