Mixed Signals

Traditionally, cytokines have been measured by radioimmunoassays (RIA) and enzyme-linked immunosorbent assays (ELISA). Unfortunately, these techniques are limited by their detection range and an inability to simultaneously measure multiple analytes. By using immunofluorescence assays (IFA) and extremely sensitive multiparameter flow cytometers, BioErgonomics' MultiFlow-IFA Multiple Cytokine Immunoassay kits overcome both of these limitations. BioErgonomics' Multiflow-IFA Multiple Cytokine Detect

Written byRamone Garcia

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BioErgonomics' Multiflow-IFA Multiple Cytokine Detection System

The MultiFlow-IFA assay system uses paramagnetic beads coated with specific monoclonal antibodies to capture desired analytes. After a short incubation period, the sample is washed in the provided IFA buffer. A fluorochrome-labeled monoclonal antibody is added to the beads with the captured analyte. The result of this second binding is that the bound analyte becomes fluorescent, with fluorescence intensity directly proportional to the sample analyte concentration.

One advantage of this assay system is that reporter antibodies are conjugated to fluorophores of differing emission spectra, allowing rapid and simultaneous quantification of multiple analytes. ...

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