The first automated, web-based application that enables routine use of enzymatic digestions to improve N-glycan structural analyses, GlycanAnalyzer, has been released from New England Biolabs (NEB). This software not only saves precious time for glycoanalysts who are more attuned to traditional methods, but also provides the most accurate interpretation of exoglycosidase digestion data on the market.
N-glycan structure plays a critical role in numerous physiological and pathological processes, making N-glycan structural profiling imperative to biotherapeutic development and quality control. Exoglycosidases can be used to significantly improve the accuracy of N-glycan structure determination using UPLC-FLR-MS analysis. However, interpretation of such analyses, until now, has been performed manually and can take anywhere from several days to several weeks to complete.
The new GlycanAnalyzer software pattern matches UPLC-FLR-MS peak shifts of released N-glycans following exoglycosidase digestions and automates structure assignments, which no other commercial offerings can do at the moment. It then presents ...
