The assay setup used by the J. Paul Robinson lab at Purdue University Cytometry Laboratories to screen compounds for mitochondrial toxicity. PURDUE UNIVERSITY, MARK SIMONS
In the world of high-throughput screening, it’s all about efficiency: how many compounds can you test, at what cost, and using how little in the way of materials? In such an environment, cell-free and cell-based microtiter plate assays, such as ELISA and cell imaging, typically hold sway, as these are relatively cheap, low-volume, and amenable to automation.
One technology that has not usually been associated with high-throughput screening is flow cytometry, which traditionally has been long on content—the amount of data points possible per cell—but short on throughput—the number of samples that can be run in a day. But that, says J. Paul Robinson, director of Purdue University Cytometry Laboratories, is changing. As a screening platform, Robinson says, flow cytometry “is starting to gain some ...
