Microarray enthusiasts take note: It is now possible to study relative changes in the expression levels of hundreds of proteins simultaneously thanks to the new PowerBlot Western Array Screening Service offered by BD Transduction Laboratories, a division of BD Biosciences located in Lexington, Ky. With an approach that complements the current nucleic acid methods for monitoring gene expression, this novel service analyzes cellular changes on the protein level and provides a powerful tool for proteomic studies.
Determining the effect of a certain treatment, drug, product, or disease state on cellular protein expression is as simple as sending a total cell or tissue lysate sample prepared from both control and experimental populations to BD Transduction Labs. Western Array Screening entails polyacrylamide gel electrophoresis, western blotting, and protein visualization via chemiluminescence. According to Jennifer Stevenson, manager of custom services, simultaneous screening of samples with more than 700 monoclonal antibodies (mAbs) is possible. ...
