Off-target Effects in CRISPR-Cas9 Genome Editing: Securing Specificity from thescientistllc on Vimeo.

A cause for concern regarding the popular CRISPR-Cas9 technology is the potential occurrence of off-target effects. RNA-guided Cas9 may cleave DNA sequences that are not exact complements of the guide strand when either strand harbors bulges due to insertions or deletions. Undoubtedly, a better understanding of the specificity of the CRISPR-Cas9 system can help develop strategies to minimize off-target cleavage. To take a closer look at the specificity and possible causes of off-target effects in the CRISPR-Cas9 system, The Scientist is bringing together researchers who will summarize their work on off-target effects limiting the applications of Cas9-mediated genome modification and discuss strategies for predicting and preventing such off-target effects. Attendees will have the unique opportunity of asking experts about their experience with off-target effects of the CRISPR-Cas9 system.

Topics to be covered:

  • Whole genome sequencing to assess...

Meet the Speakers:

Lauryl Nutter, PhD
Associate Director, Model Production and Cryopreservation & Recovery
The Centre for Phenogenomics
The Hospital for Sick Children

David J. Segal, PhD
Professor, Genome Center, Biochemistry and Molecular Medicine
Pharmacology, and MIND Institute
University of California, Davis


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