In the first published real use of high-throughput RNAi screening in a laboratory setting, a group of researchers at the University of California, San Francisco, has identified novel negative and positive regulators of a
Edan Foley and Patrick O'Farrell report their study of
The authors identified numerous components of signal transduction, including negative and positive regulators of innate immune signaling, a hierarchy of gene action, and a novel gene,
"These double-stranded RNA messages are really powerful—and particularly powerful and easy to use in Dropsophila," O'Farrell said. "We can make any double-stranded RNA, pretty much, and we just pipette it on ...