Multiomic cytometry, powered by Feature Barcode technology, allows ultra-high parameter cell phenotyping using both protein and gene expression readouts. By employing oligo-conjugated antibodies, it is possible to profile a virtually unlimited number of cell surface epitopes and couple that information with whole transcriptome or targeted mRNA sequencing data on an individual cell level. Cell surface epitope information can also be paired with full-length V(D)J sequences from the same lymphocyte.
This Technique Talk will provide an overview of multiomic cytometry and highlight the best practices for sample preparation and experimental design for multiomic single cell experiments.
- Comparing various cytometry technologies, including multiomic cytometry
- Exploring the experimental workflow of a multiomic single cell experiment
- Important factors to consider before designing an experiment using Feature Barcode technology
- The dos and don’ts of sample preparation
Meet the Instructor:
Dagmar Walter, PhD
Senior Scientist, Sample Prep