Courtesy of Won-Ki Huh
 LOCAL COLOR: Researchers used librar-ies of tagged proteins to determine where those proteins accumulate, and to what levels. Shown are representative localization (top) and co-localization (bottom) data.

Researchers from the University of California, San Francisco, have probed the yeast proteome in the highest level of detail to date. Using sets of fusion-tagged proteins, the scientists, led by Howard Hughes Medical Institute investigators Erin O'Shea and Jonathan Weissman, obtained abundance and cellular localization data for more than 75% of the proteins expressed in Saccharomyces cerevisiae during log-phase growth.1,2 Their results represent the first truly global view of the yeast proteome. "This is a landmark in that it's making the leap from a purely descriptive cataloging of protein expression to one that describes a genome-wide quantitative analysis," says Charlie Boone of the University of Toronto.

The researchers used homologous recombination to introduce engineered genes directly into the...

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