Researchers from the University of California, San Francisco, have probed the yeast proteome in the highest level of detail to date. Using sets of fusion-tagged proteins, the scientists, led by Howard Hughes Medical Institute investigators Erin O'Shea and Jonathan Weissman, obtained abundance and cellular localization data for more than 75% of the proteins expressed in Saccharomyces cerevisiae during log-phase growth.1,2 Their results represent the first truly global view of the yeast proteome. "This is a landmark in that it's making the leap from a purely descriptive cataloging of protein expression to one that describes a genome-wide quantitative analysis," says Charlie Boone of the University of Toronto.
The researchers used homologous recombination to introduce engineered genes directly into the genome in frame with the gene of interest. This ensured that each gene was expressed by its own promoter, making it more likely that the resulting full-length fusion proteins were expressed in ...
