RNAi: Five Tips to Better Silencing - Table 2: RNAi-Specific Viral KitsCompanyKitSize (reactions)Price (USD)Ambionwww.ambion.compSilencer Adeno 1.0-CMV System5$520pSilencer 5.1 Retro System20$250Invitrogenwww.ambion.comBLOCK-iT AdenoviralRNAi Expression System20$941BLOCK-iT InducibleLentviral RNAi System20$1594

RNAi: Five Tips to Better Silencing - Table 3: siRNA Transfection ReagentsCompany Maximum transfections/mlPrice/ml (USD)Ambionwww.ambion.comsiPORT neoFXtransfection reagent1,250$185siPORT aminetransfection reagent660$345siPORT lipidtransfection reagent1,250$210B-Bridge Internationalwww.b-bridge.comQuick Step transfection reagent

RNAi: Five Tips to Better Silencing - Table 4: Selected Online siRNA-Design ToolsDeveloperNameURLRef.AmbionsiRNA Target Finderwww.ambion.com/techlib/misc/siRNA_finder.html1DharmaconsiDESIGNwww.dharmacon.com/sidesign/2GenScriptSiRNA Construct Builderhttps://www.genscript.com/ssl-bin/app/rnai?op=known siR

Scientists generate the highest-resolution draft sequence of the domestic dog

Adult nematode worms contain 959 somatic cells, and thanks to Nobel laureate John Sulston and colleagues, scientists know the lineage of every one of them.

are a hot topic these days.

Chromatin immunoprecipitation (ChIP), or location analysis, is an antibody-based method for identifying the segments of DNA recognized by a particular transcription factor in a native chromatin environment.

G-matrix Fourier transform (GFT) NMR, a technique developed several years ago for rapid collection of nuclear magnetic resonance data, has been used to determine the structures of eight proteins in less than a month.1 "People usually work for many months for one structure. And even a year is not unusual," says coauthor Thomas Szyperski of the University of Buffalo. [see related story, page 36]The work demonstrates for the first time that GFT NMR works on a "real world" scale, meaning a protein c

While politicos continue to debate the ethics of expanding research on embryonic stem cells, the scientific debate persists as to whether adult stem cells are multipotent, or if they even need to be in order to be therapeutically relevant.

Short of sticking electrodes directly into an organism's brain, scientists looking to image neural signaling in living systems have few options.

An interdisciplinary team at Rensselaer Polytechnic Institute (RPI) in Troy, NY, has developed a computer program that uses protein structural information to predict how proteins separate on a chromatography column.1 "This is something that is usually done by trial and error, but in this case we've used information from the experiment to build a computer model that enables us to make that prediction," explains coauthor Curt Breneman, a computational chemist at RPI.The technique first takes exper

Researchers imaging DNA gels can put away their UV-protective gear.