Bio-Rad Releases Labeled Antibodies

Fluorescent Multiplex Detection of Proteins in Western Blots

Written byBio-Rad Laboratories
| 3 min read

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Bio-Rad Laboratories Inc. (NYSE: BIO and BIOb) today announced the launch of StarBright™ Blue 700, a fluorescent dye labeled secondary antibody for use in multiplex western blotting with a two- to fourfold lower limit of detection than IR800 labeled antibodies, the current industry standard. The company is also launching hFAB™ Rhodamine Anti-Housekeeping Protein Primary Antibodies for one-step detection of housekeeping proteins using western blots.

Detecting and quantifying multiple proteins simultaneously using western blotting can be challenging. Researchers are faced with performing either several time-consuming and error-prone chemiluminescent western blots or one fluorescent blot, which involves waiting up to 10 minutes to detect a signal that might be obscured by high background noise due to nonspecific binding.

To address those workflow challenges, Bio-Rad created StarBright Blue 700, a line of secondary antibodies that offer highly sensitive fluorescent detection, short exposure times, and easy multiplexing for western blotting.

The StarBright Blue ...

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