Molecular Devices, at SLAS 2015, launched a homogeneous, no-wash assay to measure ligand- and voltage-gated potassium channel activity. The FLIPR® Potassium Assay Kit uses a novel thallium-sensitive indicator dye and a masking agent to significantly widen the conventional assay window, enabling studies of channels with low conductance or low expression levels. The kit's protocol eliminates the customary wash steps that introduce artificial variation and limit high-throughput automation.
Current thallium-based potassium ion channel assays require multiple buffer or media replacements, making them slow and cumbersome, and prone to well-to-well variation. Pervasive background noise also undermines accurate assessment of the small signals generated by many low-conductance channels or low-expressing cell lines.
The FLIPR Potassium Assay Kit directly measures potassium channel function using a thallium-sensitive indicator dye that passively diffuses into cells as an acetoxymethyl ester. Cytoplasmic esterases cleave the dye into its fluorogenic form. A proprietary masking agent further reduces the background...
"The error bars are tighter with the new Molecular Devices kit, facilitating analysis of weakly expressed or weakly conducting potassium channels. The no-wash and no-buffer protocol disturbs cells less and speeds up automation," wrote one of the kit's beta testers, a principal scientist who leads pharmacology testing at one of the world's ten largest pharmaceutical companies.
About Molecular Devices, LLC
Molecular Devices is one of the world's leading providers of high-performance bioanalytical measurement systems, software and consumables for life science research, and pharmaceutical and biotherapeutic development. Included within a broad product portfolio are platforms for high-throughput screening, genomic and cellular analysis, colony selection, and microplate detection. These leading-edge products enable scientists to improve productivity and effectiveness, ultimately accelerating research and the discovery of new therapeutics. Molecular Devices is committed to the continual development of innovative solutions for life science applications. The company is headquartered in Silicon Valley, California with offices around the globe.