There is increasing interest in using insect cells as production hosts for recombinant proteins and viral vectors. Used in conjunction with the BEVS, insect cells are able to produce large quantities of proteins with complex post-translational modifications in short time-frames. These insect cells readily adapt to high-density culture and this feature, coupled with their high yields, makes these cells ideal for large-scale expression.
Yeast hydrolysate is commonly used as a nutritional supplement in insect cell culture, but there are major concerns about the inconsistent performance of cultures. There can be significant differences in growth and protein production between hydrolysate batches whereas the serum-free, low hydrolysate formula of IS Sf Insect provides less batch variation and more consistent growth and protein yields.
“Insect cell expression systems are appealing because of their suitability for large-scale, high density cell culture, and ability to produce high yields of recombinant proteins and viral vectors. However, ...
