A rapid and inexpensive fluorescence method for screening conditions that affect protein thermal stability, such as protein mutations, ligand binding, and buffer formulations (like pH, salts, detergents, and other additives).
GloMelt™ dye performs very well in high detergent concentrations, unlike SYPRO® Orange
GloMelt™ dye generates a strong signal because it is optimized for detection in the SYBR® Green channel of qPCR instruments
Great for high throughput assays, low reaction volumes and low protein concentrations
GloMelt™ dye tolerates DTT very well, unlike PROTEOSTAT® PTS dye
Can be combined with ROX to get very precise replicates in qPCR systems requiring ROX reference dye
Interested in reading more?
The Scientist ARCHIVES
Become a Member of
Receive full access to more than 35 years of archives, as well as TS Digest, digital editions of The Scientist, feature stories, and much more!