Natural killer (NK) cells, known for their ability to kill tumor cells, are promising agents for cell-based cancer immunotherapies. CRISPR-Cas9 gene editing can be used to effectively modify the genetic makeup of inducible pluripotent stem cell (iPSC)-derived NK cells towards this end, but there is an urgent need to characterize their potency and efficacy as a preclinical cancer immunotherapy. IsoPlexis' single-cell proteomics system addresses this challenge by connecting each immune cell to the many cytokines it secretes, revealing correlations to in vivo outcome across a range of disease areas.
The Polyfunctional Strength Index (PSI) delivers correlative potency data
The IsoPlexis system identifies which cells are polyfunctional (i.e., those powerful cells that secrete multiple cytokines) and quantitates the cytokine concentrations within each cell. PSI combines these two single-cell metrics to effectively identify highly potent immunotherapies.
PSI reveals cell potency of gene-edited, iPSC-derived NK cells
To study the role of cytokine-inducible SH2-containing ...
