How GFP-grabbing nanobodies enable instant tracking of protein dynamics in live cells.
LlamaTags (purple) grab cytoplasmic GFP (green), localizing it to the fused protein of interest (blue) and increasing GFP fluorescence intensity (top). In this example, the LlamaTagged protein is a transcription factor involved in patterning of the early fruit fly embryo. Recruitment of the readily available GFP to the tagged transcription factor therefore causes the nuclei containing the factor to glow brightly (bottom).
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In mammals, intracellular clocks begin to tick within days of fertilization.
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