September 2003

Scientific societies are an essential part of the research landscape. Almost all of us are members of one or more of them, and we have numerous reasons for joining. When I was a PhD student, I joined the British Society for Immunology, in part for the sense of belonging. I was eager to consider myself an immunologist; getting the badge of membership was a small but pleasurable step. My more pragmatic next-door neighbor, Steve, is a member of the American Association for the Advancement of Sci

Improving the Postdoctoral Experience Ned Shaw Editor's note: Responding to readers' concerns about treatment of postdoctoral fellows in US academic life science labs, The Scientist invited the National Postdoctoral Association to participate in an online discussion with science policy leaders. Attendees included Rita Colwell, director of the National Science Foundation; economist Richard Freeman, Harvard University; Michael Gottesman, deputy director for Intramural Research, National Insti

Click for larger version of survey graph (27K) A survey of 314 of our readers provides a picture of their science-reading habits. The majority, 56%, spends more than three hours per week reading primary research articles in print and online, and 10% read more than 10 hours per week. We asked respondents which multidisciplinary journals they read frequently. Naturally, The Scientist came out on top - these are readers of The Scientist after all - with 80% reading or skimming more than half t

Foundations | Little Green Bacteria Click for larger version of notes (68K) When I first heard Paul Brehm [then at Tufts University] mention green fluorescent protein at a seminar in the late 1980s, I got excited; I knew it had the potential to be an expression marker. I talked with Doug Prasher, who was trying to isolate a gfp cDNA, and we promised to keep in touch. But we lost track of each other after I married and went to do my sabbatical at my wife's university. In September 1992, I

First Person | Mark B. McClellan Courtesy of FDA Mark B. McClellan, the baby-faced, dual-degreed head of the Food and Drug Administration, is rarely the prince when he plays dress-up with his twin 4 1/2-year-old daughters. McClellan, the 40-year-old former Stanford economist and internist, is generally the ogre. Or the dinosaur. He takes his servile status in stride. "Princes are not an integral part of these stories," says McClellan, who was weaned on Texas politics. "There are weddings,

Science Seen | Incredible Voyager Courtesy of Parmabase  Tired of boring database interfaces? This might resemble just another cellular diagram, but it's actually a navigation device for Pharmabase, a physiology and pharmacology database (www.pharmabase.org). Although still under construction, users eventually will be able to click on any organelle and go directly to a site that will provide its relevant pharmacological properties. function sendData() { document.frm.pathName.value =

5-Prime | Getting Tidy: Protein Folding What is protein folding? It is the process by which proteins acquire their functional, preordained, three-dimensional structure after they emerge, as linear polymers of amino acids, from the ribosome. Who discovered it? In the 1940s, Linus Pauling and Robert Corey elucidated the a-helix and the b-sheet, which are considered the two fundamental building blocks of all protein secondary structures. In the early 1970s, Christian Anfinsen showed that a

Off The Cuff | Science Sybils What journal and article titles will PubMed list in 2053? "Nano-Molbiol Techniques." G.R. Kantharaj, Don Bosco College, Bangalore, India "Human genes responsible for immortality." Hari Har Joshi, Tribhuvan University, Teaching Hospital, Kathmandu "First functional interface between a (vertebrate) brain and a micoprocessor." Stephan Schuster, Max-Planck Institute, Germany "The rapid generation of speeds greater than the speed of light in space travel."

Protein folding raises some of biology's greatest theoretical challenges. It also lies at the root of many diseases. For example, the fundamental question of whether a protein's final tertiary conformation, sometimes called the native state, can be predicted from its primary amino acid sequence is also of vital importance in understanding the protein's potential capacity to form disease-inducing aggregates. MISS A FOLD, PROMPT A DISEASE Here's a list of protein folding-related disease catego

Click to view a PDF of antimicrobial peptides in various living organisms (141K) Living organisms produce a vast array of germ-killing peptides as their first-line of defense against infection. Scientists began to learn of these molecules in the 1980s from studies by Hans Boman and colleagues at the University of Stockholm, Sweden. They found that silkworm moth pupae secrete certain peptides that destroy invading bacteria. These controversial peptides (see Antibiotic Arms Race Heats Up) are

© 2002 Wiley Periodicals, Inc.  AT DEATH'S DOOR: Negatively stained Pseu-domonas aeruginosa (A) untreated, (B) treated with amphipathic a helical lytic peptide dia-stereomer (containing both L- and D-amino acids), and (C) treated with with the all L-amino acid peptide. All were treated at 60% of their minimal inhibitory concentration (MIC). At or above the MIC, significant lysis occurs (not shown). (Y. Shai, "Mode of action of membrane active antimicrobial peptides," Biopolymers (Petp

A TENTATIVE INTERPRETATION:Genomic actions of steroid hormones...Click for larger version (42K) Molecular biologists are rewriting the textbook explanation of steroid action. For 40 years, evidence has accumulated that some of the hormonally induced effects seemed too rapid for the classic model, in which steroids activate cytosolic receptors to modulate transcription. This evidence casts doubt on the so-called genomic pathway as the sole mode of steroid action. Increasing research now highl

Courtesy of Elsevier  DEADLY SPREAD: A: an insulinoma (Ins) in a Rip1Tag2 transgenic mouse. LYVE-1 immunohistochemistry demonstrates the presence of lymphatic vessels in connective tissue, but not near islets of Langerhans. B: Rip1Tag2 mice were crossed with mice which overexpress VEGF-C in pancreatic b-cells. C: An insulinoma cell breaks through a lymphatic vessel. D: An intralymphatic tumor cell mass forms. E: In a lymph node, lymphocytes (L) are surrounded by tumor cells (T). F: Immunof

Research Briefs Eating for Two, or an Entire Lineage; Serine at the Start of Life; Interdisciplinary Research Eating for Two, or an Entire Lineage Courtesy of Randy Jirtle Duke University researchers give a new twist to the old adage, "You are what you eat." By feeding female agouti (Avy) mice methyl-rich supplements such as folic acid and vitamin B12, Randy Jirtle and Robert Waterland reduced agouti gene expression in their offspring. This change, caused by direct methylation of a transp

Front Page Tracking and Archiving PDFs; Clasp that Cover Slip; Electrifying Gene Therapy SOFTWARE WATCH | Tracking and Archiving PDFs When Martin Kucej, a molecular biology postdoctoral fellow at Comenius University in Bratislava, Slovakia, couldn't find a journal article on his shelf of documents, he decided to digitize all the documents pertaining to his research project. But he could not find a program that would allow him to do that and share the library with his laboratory colleagues,

Courtesy of Andrew Gooley, Proteome Systems Among proteomics techniques, protein microarrays may be getting a lot of buzz these days, but two-dimensional gels still do most of the work. That's because protein arrays present a number of technical challenges that have limited their implementation.1 Proteins vary wildly in stability, solubility, viscosity, and ease of synthesis, for instance. And, the in vitro translated proteins generally used to construct protein biochips often lack the wide d

Courtesy of Agilent Technologies With last year's complete sequencing of the human genome, a number of manufacturers raced to produce the first series of human genome microarrays, which were actually sets of two or more chips or slides. The next generation of human whole-genome arrays places the entire genome on a single chip, allowing scientists to observe gene expression profiles on one surface and under uniform experimental conditions, minimizing sample use and labor. "Now that the human g

Courtesy of Q3DM Drug discovery companies frequently use high-throughput cell imaging systems to increase the efficiency of secondary screening of drug candidates, and the number of available systems has grown in recent months to meet this demand. Among the competitors is San Diego-based Q3DM's EIDAQ 100 system, the product of a combination of patented technologies that, according to the company, improve throughput and image fidelity over conventional automated cell-imaging systems. The EIDA

TIP TROVE | When Postdocs Change Their Minds Courtesy of Gordon Keller If you're not excited about what you're doing, you're just not going to do it well. Mentors should encourage independent thinking. If postdocs want to change the direction of their projects, or explore new avenues, I certainly encourage it. However, if you find that you've chosen an area of research that doesn't interest you, I would advise you to think carefully about your choices ... and if you are absolutely certain y

File Photo The European Molecular Biology Organization (EMBO) is setting up a grant program to fund ¤30 million each year for outstanding research in molecular biology. The program may be of particular interest to scientists from smaller countries and to those who are working in research that is difficult to fund (such as work with genetically modified plants). This scheme may make science more competitive because researchers will vie for grants with peers from all over Europe, rather th

Courtesy of Robert Blanchard Early Indications: As an undergraduate I was attracted to experimental psychology, because it seemed to provide a scientific approach to understanding animal and human behavior. Pivotal Papers: There were two; one in each area that has turned out to be a focus of the lab. "Crouching as an Index of Fear" (J Comp Physiol Psychol, 67:370-5, 1969) raised the heretical specter of a powerful unconditioned fear response and also provided a neat demonstration of rapid a

Courtesy of Carol L. Manahan Recently, I attended a symposium called "Catalyzing Team Science," which gathered representatives from the institutes of the National Institutes of Health; it was sponsored by the Bioengineering Consortium (BECON), a senior interagency committee that focuses on bioengineering efforts at the NIH. This was the first time that BECON has invited a postdoctoral fellow to such a symposium (www.becon1.nih.gov/symposium2003.htm). In contrast to other areas of science, suc

File Photo How can I break into the biotech or pharmaceutical industry? That's a question many of my readers ask. Michael Ferguson, senior manager of clinical development for Memphis, Tenn.-based Medtronic Sofamor Danek, offers insights for people who wish to take the industry path. Two years into his PhD studies in applied physiology at the University of Florida, Ferguson decided he wanted to join a biotechnology company or a clinical setting. His first step was to work for a small firm dur

My alarm clock was blinking an erroneous time, but I ignored it; I had just taken a much-appreciated nap, and I needed to get back to a yeast cell biology conference at Cold Spring Harbor Laboratory. I was staying 10 miles away at a Vincentian seminary that housed students like me. My fan was running, and my cellular phone recharger was pulsing red. I had no idea that the power was coming from a backup generator. I was probably one of a few people of about 50 million, from Canada to New Jers