The NEBNext RNase H-based RNA depletion protocol offers efficient and specific depletion of the abundant RNA species that interfere with the analysis of both coding and non-coding RNAs of biological significance. The newly-released NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) and NEBNext rRNA Depletion Kit (Bacteria) complement the existing NEBNext rRNA Depletion Kit (Human/Mouse/Rat). All kits are available both with and without RNA Sample Purification Beads, and have a 2-hour workflow, requiring less than 10 minutes of hands-on time. The kits also support a broad range of input amounts of total RNA, from low ng to 1 µg, and are effective with intact or degraded RNA.
The NEBNext Globin & rRNA Depletion Kit depletes adult, fetal and embryonic globin mRNA (HBA1/2, HBB, HBD, HBM, HBG1/2, HBE1, HBQ1 and HBZ), as well as cytoplasmic rRNA (5S, 5.8S, 18S, 28S, ITS and ETS) and mitochondrial rRNA (12S and 16S). "As researchers who ...
