Tecan has launched a fully automated affinity purification solution for the extraction of large biomolecules, such as proteins, antibodies and antibody-drug conjugates. This set-up takes advantage of the new Thermo Scientific™ MSIA™ Streptavidin EVO microcolumns, which have been specifically designed for use on Tecan’s proven Freedom EVO® platform. In combination with the Freedom EVO workstation, this solution is ideal for high throughput applications in biopharmaceutical development, clinical research and anti-doping laboratories.
Housed within a pipette tip, the proprietary MSIA microcolumns are densely coated with streptavidin for affinity purification using any biotinylated ligand, even at low concentrations. Compared to traditional resin- and bead-based methods, this new approach offers improved analytical sensitivity and lower background noise, while the automated workflow enables easier method standardization and transfer between labs. This allows multi-analyte characterization over a wide dynamic range of three orders of magnitude, while maintaining good reproducibility of <5 %CV*.
Preeti Saini, Product ...
