The paper:
H. Feng, et al., “Clonal integration of a polyomavirus in human Merkel cell carcinoma,” Science, 319:1096–100, 2008. (Cited 163 times)
The finding:
When the husband and wife team of Yuan Chang and Patrick Moore started looking for the causes of Merkel cell carcinoma (MCC), a rare skin cancer, they suspected a virus. They had discovered that Kaposi’s sarcoma was caused by a herpesvirus 16 years ago, and both Kaposi’s and MCC occur more often in immunosuppressed patients. Once again, they found a new type of polyomavirus present in 8 of 10 MCC samples tested.
The method: Yang and Moore spent 10 years developing a technique known as digital transcriptome subtraction, which they used to detect the virus. They sequenced the complete cDNAs of four MCC tumors and isolated sequences that didn’t align with the human genome, says Moore. They then compared those 2,400 isolated sequences against virus genomes, ...
