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JFT1 has reacted with hydrogen peroxide and APEX2 to create red fluorescence near APEX2 that remains separate from the green mitochondria.
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A modified fluorescent protein scheme survives harsh electron microscopy conditions, offering new solutions for dual imaging.

fluorescent protein

To flag neurons that have experienced genotoxic stress, researchers developed an in vivo sensor using an adeno-associated viral vector, called PRISM. Because a cell’s DNA damage response (DDR)—which activates in response to stressors such as environmental toxins or the buildup of misfolded proteins—also responds to invading pathogens, PRISM has an easier time transfecting cells whose damage response mechanisms are preoccupied with existing DNA damage. Once inside, the virus hijacks the neuron’s DNA replication machinery, which reverts an engineered frameshift mutation in the virus and thereby prompts the production of a fluorescent protein that can be observed via microscopy.

Infographic: DNA Damage Viewed with Unprecedented Clarity

Broken DNA

DNA Damage Viewed with Unprecedented Clarity

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Essential Genes Are Dominantly Activated by Single Transcription Factors

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Rethinking ALS Biomarkers: From Discovery to Clinical Impact

Rethinking ALS Biomarkers: From Discovery to Clinical Impact

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The Scientist Digest cover September 2026
September 2026

Multiplex Microscopy Becomes Easier with Encoded Antibodies

A new system that enables researchers to uniquely tag monoclonal antibodies for use in microscopy could help simplify complex imaging studies.

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Essential Genes Are Dominantly Activated by Single Transcription Factors

Essential Genes Are Dominantly Activated by Single Transcription Factors

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Rethinking ALS Biomarkers: From Discovery to Clinical Impact

Rethinking ALS Biomarkers: From Discovery to Clinical Impact

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Engineering CAR-Neutrophils In Vivo to Target Glioblastoma

Engineering CAR-Neutrophils In Vivo to Target Glioblastoma

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