From CRISPR to Prime Editing: The Evolution of the Genome Editing Revolution
From CRISPR to Prime Editing: The Evolution of the Genome Editing Revolution
Even as CRISPR-based tools become a lab staple, scientists strive to tackle the associated technological challenges to improve their efficacy and safety.
Even as CRISPR-based tools become a lab staple, scientists strive to tackle the associated technological challenges to improve their efficacy and safety.
prime editing
A Gene Editing System Corrects Cystic Fibrosis Mutation
RJ Mackenzie | Sep 4, 2024 | 4 min read
An optimized version of prime editing technology raises the possibility of a one-time treatment for cystic fibrosis.
A Prime-Editing Based Approach Records Cellular Genetic History
Maggie Chen | Aug 30, 2024 | 3 min read
Researchers developed a technique, ENGRAM, to keep a running log of the elements that control gene expression in cells.
Measuring Mutagenesis with Precision Genome Editing
Deanna MacNeil, PhD | May 3, 2024 | 5 min read
A new prime editing sensor library targeting p53 mutations allowed scientists to interrogate thousands of tumor genotypes as they arose in endogenous contexts.
Prime Time Precision with CRISPR Technologies
The Scientist | Apr 29, 2024 | 1 min read
Base editors and prime editors help researchers perform more precise in vivo and ex vivo translational research.
Delivering Prime Editors With Virus-like Particles
Deanna MacNeil, PhD | Mar 11, 2024 | 3 min read
An iterative engineering approach to improve prime editor delivery helped scientists correct genetic vision defects in mice.
Genotoxic Effects of Base and Prime Editing
Kamal Nahas, PhD | Jan 12, 2024 | 5 min read
A risk-benefit analysis of gene editing tools in stem cells revealed that base and prime editing carry vulnerabilities similar to those of CRISPR-Cas9, but at a reduced rate.
CRISPR Gene Editing: Cas9 and Beyond
Johanna Pruller, PhD and Deanna MacNeil, PhD | Nov 29, 2023 | 7 min read
Researchers create and improve CRISPR-Cas mediated gene editing technologies based on prokaryotic CRISPR systems and eukaryotic DNA repair mechanisms.
Can “Gene Writing” Deliver What Gene Editing Can’t?
Dan Robitzski | Dec 12, 2022 | 10+ min read
A biotech startup called Tessera Therapeutics has made a splash with its claims about the trademarked technology. Is the excitement justified?
Infographic: Two Guide RNAs Make for Large, Stable Insertions
Dan Robitzski | Jun 13, 2022 | 1 min read
A new technique goes beyond CRISPR and writes two complementary strands of DNA directly into the genome.
With Two RNA Guides, Editing Technique Writes Long DNA Insertions
Dan Robitzski | Jun 13, 2022 | 3 min read
The approach, called GRAND, uses a second guide RNA to write complementary strands of DNA in targeted genomic locations, resulting in efficient insertions that can be hundreds of base pairs long.
New “Prime Editing” Method Makes Only Single-Stranded DNA Cuts
Emma Yasinski | Oct 21, 2019 | 4 min read
In demonstrations in cell lines, the technique has a similar efficacy to CRISPR-Cas9, but fewer off-target effects.