Two prime editing guide RNAs (pegRNAs), deliver Cas9 enzymes to targeted regions of the genome.
Infographic: Two Guide RNAs Make for Large, Stable Insertions
A new technique goes beyond CRISPR and writes two complementary strands of DNA directly into the genome.
Infographic: Two Guide RNAs Make for Large, Stable Insertions
Infographic: Two Guide RNAs Make for Large, Stable Insertions

A new technique goes beyond CRISPR and writes two complementary strands of DNA directly into the genome.

A new technique goes beyond CRISPR and writes two complementary strands of DNA directly into the genome.

prime editing
Genetic Syndrome and Genetic Disorder, 3D illustration of science concept.
With Two RNA Guides, Editing Technique Writes Long DNA Insertions
Dan Robitzski | Jun 13, 2022 | 4 min read
The approach, called GRAND, uses a second guide RNA to write complementary strands of DNA in targeted genomic locations, resulting in efficient insertions that can be hundreds of base pairs long.
prime editing crispr cas9 genome editing techniques
New “Prime Editing” Method Makes Only Single-Stranded DNA Cuts
Emma Yasinski | Oct 21, 2019 | 4 min read
In demonstrations in cell lines, the technique has a similar efficacy to CRISPR-Cas9, but fewer off-target effects.