CRISPR-Cas technology has radically changed how transgenic models are created. Through CRISPR approaches, researchers have pioneered how to create complex animal models, including long cassette knock-ins and conditional knockout mouse models. Techniques such as Easi (Efficient Additions with ssDNA Inserts)-CRISPR and GONAD (Genome editing via Oviductal Nucleic Acids Delivery) have become prevalent for animal transgenesis.
This Technique Talk will offer a historical overview of mouse transgenic technologies and discuss how CRISPR-Cas has redefined these traditional gold standards to give scientists more flexibility and capability than ever before.
Meet the Instructor:
Channabasavaiah B. Gurumurthy, MVSC, PhD
Director, Mouse Genome Engineering Core Facility
Professor of Pharmacology and Experimental Neuroscience
University of Nebraska Medical Center