OXFORD UNIVERSITY PRESS, AUGUST 2016When Technologies Meet
Developing new approaches for getting ES or iPS cells to develop into complex 3D structures is one important aspect of stem-cell technology. An equally crucial one is the ability to control the differentiation pathway. At the heart of such control is an understanding of the genetic pathways underlying the development of specific cell types, tissues, and organs in the normal developing embryo. Just as important is having the ability to manipulate such pathways in a culture dish. And it’s here that a combination of genome editing and stem-cell technology is proving particularly fertile. In particular, the flexibility and efficiency of approaches like CRISPR-Cas9 are taking the genetic manipulation of ES and iPS cells, and the differentiated cells derived from them, to a previously undreamt of ...
