WIKIMEDIA, KLINTWORTH GK
Human endothelial cells that make up the lining of blood vessels can be genetically altered using the CRISPR/Cas9 system, according to a study published today (May 4) in Circulation Research.
In addition to animal models, human endothelial cells grown in tissue culture provide a valuable model to understand the biology of blood vessels. Additionally, research using the cells may one day contribute to the development of methods to heal tissues by engineering them in the lab. However, because the cells are already highly specialized, they have been difficult to manipulate genetically to probe the functions of particular genes in biological processes.
In the first reported use of the CRISPR/Cas9 technique in human endothelial cells, researchers from the Yale University School of Medicine infected cultured endothelial ...
