Bacterial artificial chromosome (BAC) systems offer numerous benefits. These roomy vectors can contain an entire gene and all of its associated regulatory elements, allowing scientists to easily manipulate gene expression. In addition to housing large genes, BACs facilitate de novo sequencing projects in a number of species, from microbes to humans. However, before you can use BAC systems to fulfill your DNA sequencing needs, you must first run the necessary quality control experiments. These essential assays guarantee that your gene of interest and all of its machinery successfully inserted into the BAC clones.
For studies using BAC systems, researchers often use pulsed-field gel electrophoresis (PFGE) to separate DNA fragments. Unfortunately, PFGE takes a long time to run. Clocking in at around 16 hours, it is far from a quick quality check. When used alongside the Agilent Technologies pulsed-field capillary electrophoresis Femto Pulse system, the new 165 kb BAC Analysis kit ...
