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Two prime editing guide RNAs (pegRNAs), deliver Cas9 enzymes to targeted regions of the genome.
Infographic: Two Guide RNAs Make for Large, Stable Insertions
A new technique goes beyond CRISPR and writes two complementary strands of DNA directly into the genome.
Infographic: Two Guide RNAs Make for Large, Stable Insertions
Infographic: Two Guide RNAs Make for Large, Stable Insertions

A new technique goes beyond CRISPR and writes two complementary strands of DNA directly into the genome.

A new technique goes beyond CRISPR and writes two complementary strands of DNA directly into the genome.

Cas9
Genetic Syndrome and Genetic Disorder, 3D illustration of science concept.
With Two RNA Guides, Editing Technique Writes Long DNA Insertions
Dan Robitzski | Jun 13, 2022 | 3 min read
The approach, called GRAND, uses a second guide RNA to write complementary strands of DNA in targeted genomic locations, resulting in efficient insertions that can be hundreds of base pairs long.
A cockroach clings to the inside of a white mug.
Injecting Cockroaches with CRISPR Gene Edits Their Offspring
Sophie Fessl, PhD | May 25, 2022 | 4 min read
A new method has allowed researchers to conduct the first gene knock-out and knock-in edits on cockroaches and may extend to many other insects.
3D representation of a DNA helix and with a base offset from the main helix, illustrating the concept of base editing.
A CRISPR Alternative for Correcting Mutations That Sensitize Cells to DNA Damage
Deanna MacNeil, PhD | 3 min read
Researchers turned to base editors to correct mutations causing the rare genetic disease Fanconi anemia without inducing double-strand DNA breaks.
cartoon depiction of a pair of scissors about to cut a DNA double helix
Researchers Uncover New Families of Gene-Editing Enzymes
Annie Melchor | Sep 15, 2021 | 2 min read
The results reveal evolutionary relatives of the Cas9 enzyme now used extensively in biotechnology.
cartoon showing a hand taking a tool to a double-helix of DNA to represent gene editing
WHO Releases New Recommendations on Human Genome Editing
Annie Melchor | Jul 12, 2021 | 3 min read
The guidance comes after two years of consulting with hundreds of stakeholders, including indigenous peoples, religious leaders, patient groups, and scientists.
Fluorescent microscopy images of cells after being transfected.
Universal Transfection Reagents: Improving Efficiency and Decreasing Cell Toxicity
The Scientist Creative Services Team, MilliporeSigma, and Roche | 4 min read
Researchers optimize their transfection protocols with the ideal transfection reagent that has multiple applications, low cytotoxicity, and high transfection efficiency.
feluda cas9 fncas9 covid-19 sars-cov-2 diagnostics coronavirus pandemic india
Assay for Sickle Cell Anemia Is Repurposed to Diagnose COVID-19
G.B.S.N.P. Varma | Nov 18, 2020 | 5 min read
A CRISPR-based, paper-strip test developed by Indian researchers performs as well as real-time PCR in identifying the presence of SARS-CoV-2 and returns results within an hour.
CRISPR, Cas-9, Nobel, Chemistry
CRISPR’s Adaptation to Genome Editing Earns Chemistry Nobel
Amanda Heidt | Oct 7, 2020 | 4 min read
Emmanuelle Charpentier and Jennifer Doudna reprogrammed the bacterial immune response into one of the most popular tools for genetics and molecular biology.
Symptoms in ALS Mouse Model Improve with CRISPR Base Editing
Abby Olena, PhD | Apr 10, 2020 | 4 min read
Researchers slowed disease progression in the mice by injecting two different viral vectors, each containing one part of the DNA encoding the Cas9 protein, to edit the causative gene.
prime editing crispr cas9 genome editing techniques
New “Prime Editing” Method Makes Only Single-Stranded DNA Cuts
Emma Yasinski | Oct 21, 2019 | 4 min read
In demonstrations in cell lines, the technique has a similar efficacy to CRISPR-Cas9, but fewer off-target effects.
First Successful Gene Drive in Mammals
Abby Olena, PhD | Jan 23, 2019 | 4 min read
Researchers use a CRISPR-Cas9 strategy to expand a desired trait from 50 percent of mouse pups to about 72 percent.
New Technique Limits CRISPR-Cas9 Off-Target Mutations
Abby Olena, PhD | Sep 12, 2018 | 3 min read
A mouse study details a method called VIVO that predicts the accuracy of any guide RNA.
New Methods to Detect CRISPR Off-Target Mutations
Sandeep Ravindran | Mar 1, 2018 | 7 min read
Researchers have developed a variety of techniques to detect when CRISPR misses the mark.
Most Accurate CRISPR Gene Editing Yet
Kerry Grens | Sep 22, 2017 | 2 min read
A tweaked Cas9 nuclease reduces off-target effects to levels below that of previous versions of the enzyme.
CRISPR Corrects RNA-based Disease Defects
Kerry Grens | Aug 10, 2017 | 2 min read
In human cells, researchers deploy the genome editor to snip out toxic repetitive sequences.
Details Published on CRISPR-treated Embryos
Kerry Grens | Aug 2, 2017 | 4 min read
Scientists correct a mutation in fertilized eggs that causes a severe cardiac disease.
Scientists Destroy Entire Chromosome with CRISPR
Kerry Grens | Aug 1, 2017 | 1 min read
Multiple DNA breaks at either the centromere or the long arm of the mouse Y chromosome cause it to fragment and disappear.
Human Genetic Variation May Complicate CRISPR
Ashley Yeager | Jul 31, 2017 | 2 min read
Slight sequence differences confound target sites in precision genome-editing, a study shows.
Anti-CRISPR Protein Reduces Off-Target Effects
Diana Kwon | Jul 12, 2017 | 3 min read
AcrIIA4, an inhibitor protein from the Listeria bacteriophage, can block DNA from binding to Cas9 during genome editing.
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