Translational research requires extensive preclinical development. During these early stages, scientists use cell cultures extensively to test and validate a broad range of research questions before moving to animal and human models. As such, in vitro models are essential tools for advancing both basic and applied science. While the protocols for growing different cell types vary widely, all cell cultures have one critical consideration in common: the need to mitigate contamination.
Scientists use various approaches to safeguard against contaminants, including using aseptic techniques, adding antimicrobial agents to cell cultures, filtering reagents, and routinely screening cultures. However, despite best efforts, cell cultures become infected, affecting cell viability and a range of other biochemical and biomolecular pathways. When this happens, scientists must often discard the cells and start anew, wasting precious resources and time.
Mycoplasma—a sub-micron sized bacterium without a cell wall—is a particularly formidable enemy because it is pervasive, resistant to ...
