CRISPR guide RNAs target specific spots in the genome for the Cas9 enzyme to cut, forming a double-strand break. A machine learning algorithm predicts which types of repairs will be made at a site targeted by a specific guide RNA. Possibilities include an insertion of a single base pair (a), a small deletion (b), or a larger change known as a microhomology deletion (c).

© Kelly Finan

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May 2019 The Scientist Issue

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