Scientists Destroy Entire Chromosome with CRISPR

Multiple DNA breaks at either the centromere or the long arm of the mouse Y chromosome cause it to fragment and disappear.

kerry grens
| 1 min read

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ISTOCK, POLESNOYResearchers have managed to wipe out an entire mouse chromosome using CRISPR-Cas9. They aimed numerous double-strand breaks at either the centromere or the long arm of the Y chromosome, causing it to fragment and become deleted from XY mouse embryonic stem cells in vitro.

The centromere-targeting approach also resulted in Y-chromosome loss when it was applied to mouse embryos in vivo.

“This study shows that targeted chromosome deletion is achievable and relatively efficient both in vitro and in vivo using CRISPR/Cas genome editing,” the authors, based at the University of Adelaide in Australia, wrote in their report, which appears in the August issue of Molecular Therapy.

The scientists set out to explore whether CRISPR-Cas9 genome editing could be applied to aneuploidies, conditions caused by extra chromosomes. They first cut the centromere of the Y chromosome in 41 places in vitro, and found that in 90 percent of cells the Y chromosome was undetectable, compared to 13 percent in untreated cells. Similarly, cutting the long arm of the Y chromosome ...

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Meet the Author

  • kerry grens

    Kerry Grens

    Kerry served as The Scientist’s news director until 2021. Before joining The Scientist in 2013, she was a stringer for Reuters Health, the senior health and science reporter at WHYY in Philadelphia, and the health and science reporter at New Hampshire Public Radio. Kerry got her start in journalism as a AAAS Mass Media fellow at KUNC in Colorado. She has a master’s in biological sciences from Stanford University and a biology degree from Loyola University Chicago.

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