The image depicts a lung cancer tri-culture model made with RASTRUM Allegro. Primary normal human lung fibroblasts (NHLFs) were printed with primary human umbilical vein endothelial cells (HUVECs) and A549 lung adenocarcinoma cells using RASTRUM’s Imaging Model architecture. Cells were cultured for 7 days and stained using a PhenoVue Cell Painting Kit (Revvity). Cell nuclei (cyan) were labeled with Hoescht, filamentous actin (yellow) with phalloidin, and membrane glycoproteins (magenta) with concanavalin A.
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