To flag neurons that have experienced genotoxic stress, researchers developed an in vivo sensor using an adeno-associated viral vector, called PRISM. Because a cell’s DNA damage response (DDR)—which activates in response to stressors such as environmental toxins or the buildup of misfolded proteins—also responds to invading pathogens, PRISM has an easier time transfecting cells whose damage response mechanisms are preoccupied with existing DNA damage. Once inside, the virus hijacks the neuron’s DNA replication machinery, which reverts an engineered frameshift mutation in the virus and thereby prompts the production of a fluorescent protein that can be observed via microscopy.
Infographic: DNA Damage Viewed with Unprecedented Clarity
A new genetic sensor called PRISM makes use of a host cell’s DNA replication machinery to trigger fluorescence in neurons with damaged DNA.
Infographic: DNA Damage Viewed with Unprecedented Clarity
Infographic: DNA Damage Viewed with Unprecedented Clarity

A new genetic sensor called PRISM makes use of a host cell’s DNA replication machinery to trigger fluorescence in neurons with damaged DNA.

A new genetic sensor called PRISM makes use of a host cell’s DNA replication machinery to trigger fluorescence in neurons with damaged DNA.

transfection
Broken DNA
DNA Damage Viewed with Unprecedented Clarity
Amanda Heidt | Aug 15, 2022
A new tool called PRISM draws on virus-host interactions and a DNA repair pathway to help researchers visualize how cellular stress may contribute to neurodegenerative disease.
Discover how innovation boosts clinical manufacturing efficiency
Innovating Cell Therapy
Thermo Fisher Scientific | Aug 8, 2022
A closed and scalable electroporation system is an example of how innovation can boost clinical manufacturing efficiency.
3d render illustration of Single strand ribonucleic acid.
Message in a Bottle: Developing mRNA Therapeutics
The Scientist Creative Services Team and Mirus Bio | Jul 11, 2022
A high efficiency, low toxicity method for direct RNA delivery into cells.
Learn how to develop robust cell therapy workflows
A Faster, Scalable Electroporation System for Cell Therapies
Thermo Fisher Scientific | Jun 23, 2022
Developing cellular therapeutics with an automated transfection platform.
Technique Talk: Cell Transfection Techniques
Technique Talk: Cell Transfection Techniques
The Scientist Creative Services Team | Nov 2, 2021
Learn how to overcome common transfection challenges and optimize gene delivery.
800x560-thermo-october
A Scalable Process to Efficiently Manufacture Adeno-Associated Viral Vectors
The Scientist Creative Services Team in collaboration with Thermo Fisher Scientific | Oct 28, 2021
An optimized AAV production system allows researchers to consistently generate high virus titers.
Identifying Antibodies that Target Membrane Proteins in Their Native Conformations
The Scientist Creative Services Team in collaboration with OXGENE | May 10, 2021
A new mammalian display platform enhances antibody discovery for challenging protein targets.
Securing the Antibody Supply Chain through Recombinant Antibody Technology: A COVID-19 Case Study
The Scientist Creative Services Team in collaboration with Sartorius Corporation | Mar 29, 2021
Nicholas Hutchings will discuss how recombinant antibody technology can speed up the response to a pandemic.
Optimizing Adeno-Associated Virus (AAV) Manufacturing
The Scientist Creative Services Team in collaboration with OXGENE | Feb 16, 2021
Scientists wield nature’s power to optimize adeno-associated virus (AAV) production and maximize gene therapy safety.
Laptop displays CRISPR poster.
The Next Generation of CRISPR-Cas Systems
Nathan Ni, PhD | Feb 4, 2021
Researchers reinvent genome editing with new CRISPR-Cas systems.
Accelerating Discovery in the Age of a Pandemic
The Scientist Creative Services Team | Jan 22, 2021
An expert panel will present how synthetic biology accelerates the development of diagnostics, therapeutics, and the medicinal pipeline.
The Scientist's LabTalk - Episode 4
The Scientist Creative Services Team | Dec 11, 2020
The Past, Present, and Future of Gene Therapy: How to Scale-up Successfully
Infographic: Transfection by Precision Cell Piercing
Ruth Williams | Apr 1, 2020
A microfluidic device creates a single hole in each cell to allow efficient DNA entry without compromising viability.
Precision Membrane Puncture Enables Efficient Cell Transfection
Ruth Williams | Apr 1, 2020
A device for piercing individual holes in cell membranes allows vector-free DNA delivery while maintaining cell viability.
Entry Requirements
Nicholette Zeliadt | Sep 1, 2014
Recent developments in cell transfection and molecular delivery technologies
Moving Objects Using Sound
Kate Yandell | Jul 17, 2013
Levitating and manipulating objects using sound waves could help prevent contamination of materials.
Narrow Straits
Ruth Williams | Jul 1, 2013
Transfecting molecules into cells is as easy as one, two, squeeze.