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Versatile and Sustainable: Cell Counting for the 21^st Century

The Scientist’s Creative Services Team and DeNovix Inc. | Mar 23, 2023 | 3 min read

Discover how the latest cell counting technology is reshaping a mundane task for the future.

Discover How to Verify Plasmid Quality with the Agilent Fragment Analyzer System

Analyzing Plasmids with Automated Electrophoresis

Agilent | Mar 13, 2023 | 1 min read

Verifying plasmid quality is critical for molecular biology and biomanufacturing applications.

Learn How Researchers Make the Most of Viral Vectors for Gene Therapy

Viral Vector Platforms for Gene Therapy

The Scientist’s Creative Services Team | Feb 9, 2023 | 1 min read

In both the laboratory and clinic, scientists harness viral genetic transfer capabilities to develop gene therapies that modulate cellular function.

Learn how to develop robust cell therapy workflows

Non-viral Transfection for Cell Therapy and Beyond

Thermo Fisher Scientific | Feb 1, 2023 | 1 min read

With advancements in electroporation systems, researchers have better options for introducing nucleic acids into cells.

How to Produce Successful Viral Vectors

The Scientist’s Creative Services Team | Nov 23, 2022 | 1 min read

Learn how to be successful with transfection parameters, cell culture considerations, and assessment methodology when producing viral vectors.

Learn about establishing a Gene Therapy Manufacturing Strategy

Considerations for Gene Therapy Manufacturing Strategies

Thermo Fisher Scientific | Oct 24, 2022 | 1 min read

Discover tips for setting up and optimizing adeno-associated virus production.

Fluorescent microscopy images of cells after being transfected.

Universal Transfection Reagents: Improving Efficiency and Decreasing Cell Toxicity

The Scientist’s Creative Services Team, MilliporeSigma, and Roche | Oct 5, 2022 | 4 min read

Researchers optimize their transfection protocols with the ideal transfection reagent that has multiple applications, low cytotoxicity, and high transfection efficiency.

To flag neurons that have experienced genotoxic stress, researchers developed an in vivo sensor using an adeno-associated viral vector, called PRISM. Because a cell’s DNA damage response (DDR)—which activates in response to stressors such as environmental toxins or the buildup of misfolded proteins—also responds to invading pathogens, PRISM has an easier time transfecting cells whose damage response mechanisms are preoccupied with existing DNA damage. Once inside, the virus hijacks the neuron’s DNA replication machinery, which reverts an engineered frameshift mutation in the virus and thereby prompts the production of a fluorescent protein that can be observed via microscopy.

Infographic: DNA Damage Viewed with Unprecedented Clarity

Amanda Heidt | Aug 15, 2022 | 2 min read

A new genetic sensor called PRISM makes use of a host cell’s DNA replication machinery to trigger fluorescence in neurons with damaged DNA.

DNA Damage Viewed with Unprecedented Clarity

Amanda Heidt | Aug 15, 2022 | 3 min read

A new tool called PRISM draws on virus-host interactions and a DNA repair pathway to help researchers visualize how cellular stress may contribute to neurodegenerative disease.