© WHITNEY SALGADO
By the time you read this article, another boatload of CRISPR tools will have washed up on shore. They will need testing and refinement. Nevertheless, if you ask developers whether you should take these proof-of-principle studies and try them out, you’ll only receive encouragement. After all, if it’s not yet available from Addgene, it soon will be.
The latest wave of published tools includes new lineage-tracing methods, which can provide a window into the earliest stages of development. New Cas9 inhibitors are now available for the most popular Streptococcus pyogenes Cas9 endonucleases, for use in a variety of scenarios to prevent off-target effects. Others strategies merge CRISPR-Cas9 systems with cutting-edge single-cell sequencing technologies. And researchers are, in parallel, moving beyond Cas9 to come up with ...
